Many family firms have the vision to succeed across generations to come. This means that they need to develop sustainable renewal capabilities including an innovative, aggressive, autonomous, proactive, and risk taking mindset to face future competitive demands, i.e. possess an entrepreneurial orientation (EO) towards their business activities. Combining theoretical frameworks of corporate entrepreneurship and literature on organizational cultures, our purpose is to analyze the role of culture as a family influenced resource for understanding how EO is developed over time in family businesses. We focus on how the development of two EO dimensions – autonomy and proactiveness – are fostered over time and across generations or hampered by the family influenced organizational cultures.