Governmental policies in many countries are designed to boost and support entrepreneurship in peripheral regions (Lerner 2009). This paper analyzes the perceptions about entrepreneurship framework conditions (Levie & Autio 2008) at regional level in Chile. We applied one of the Global Entrepreneurship Monitor’s methodologies, the National Experts Survey, to a sample of 695 key informants in eight regions of which six are classified as peripheral. Using non-parametric statistics we compare the differences between peripheral and core-central regions. The main results indicate that peripherally located experts perceive their regions as in a worse position than centrally located entrepreneurship experts in terms of finance access and physical infrastructure. However, the results surprisingly indicate that peripheral entrepreneurship experts are more likely to perceive general policy and government programs as being more pro-entrepreneurship, and to detect more market dynamism in their regions. This research revives the debate about specific regional policies designed to foster local new business creation, and the entrepreneurial framework conditions needed at regional level for emerging regions such as in Latin America.