Strategic Entrepreneurship (Ireland, Hitt & Sirmon, 2003) represents an important framework for integrating entrepreneurship (opportunity-seeking) and strategic (advantage-seeking) perspectives. In this context, entrepreneurial culture (EC), which consists of shared values and beliefs within an organization that support entrepreneurial behaviors, has been viewed as strategically instrumental; that is, mainly as an antecedent dimension towards sustainable competitive advantage. However, our assessment of entrepreneurs and the culture of entrepreneurial firms suggested there may be other, non-strategic aspects and motivations that characterize EC. In this paper we present a study that explored EC and revealed characteristics that supported and extended existing views on the concept.