Entrepreneurial Orientation (EO) describes a firm’s proclivity to undertake entrepreneurial ventures and has been shown to lead to better firm performance across a variety of contexts. While EO is broadly reflected in a firm’s management style, processes and practices, extant research commonly views firms that simultaneously exhibit proactiveness, innovativeness and risk-taking as having an EO. With this study, we extend existing research by providing an integrated view of the antecedent effects of national, industry and organizational culture on the level of EO of the firm. We contribute to answering the question whether business leaders can leverage organizational culture to enhance their firm’s disposition to behave entrepreneurially, or whether they are primarily subject to the influence of industry- and national culture.