Entrepreneurs face continuing challenges in achieving a satisfying balance between work and family roles due to heavy workloads and high levels of risk. As key architects of both their work and family roles, entrepreneurs can achieve balance by altering role content through role restructuring strategies, such as scaling back or role reduction, eliminating a role, expanding a role, or delegation or role sharing. Entrepreneurs who choose role restructuring strategies consistent with their level of work and family salience are also expected to achieve stronger venture performance. Our research questions are: Which role restructuring strategies promote work-family balance and strong venture performance in a demanding, resource-intensive industry such as biotechnology? What impact does gender have?