Although an increasing number of scholars are investigating the role of self-regulatory foci in entrepreneurship, progress has been hindered by important methodological challenges. Many studies have relied on measures framed in terms of one’s early upbringing and education. These measures have worked reasonably well in educational contexts. But when applied in entrepreneurship research, with adult respondents engaged in a broad diversity of affectivelyintense and personally-significant activities, with uncertain outcomes, empirical results have proven somewhat more elusive. Among the most troubling issues, many studies have suffered from low levels of validity and reliability, leading to weak statistical support for the theorized relationships. The net result is that entrepreneurship studies of self-regulation form a theoreticallyappealing but empirically-unproven body of work. To address these issues, we developed and validated a new, entrepreneurship-specific instrument for measuring individual differences in self-regulation foci.