An extensive literature has emerged in regional studies linking organization-based measures of entrepreneurship (e.g., self-employment, new start-ups) to regional economic performance (see for a review van Praag and Versloot, 2008). A limitation of the extant literature is that the measurement of entrepreneurship is not able to incorporate broader conceptual views, such as behavior, of what actually constitutes entrepreneurship. This paper fills this gap by linking the underlying and also more fundamental and encompassing entrepreneurship culture of regions to regional economic performance.